Seen as a huge underdog, Dubois floored Usyk in the fifth round in Poland but it was deemed a low blow and Usyk was given almost four minutes to recover.
The Ukrainian, 36, then won by knockout in the ninth round but Dubois said "this wrong needs to be corrected".
"This needs to go further, it needs to be pushed," the 25-year-old told BBC Sport's Ade Adedoyin.
"I should be a world champion right now. It wasn't justice.
"It needs to be called out and go further than living this lie. They just cheated out there."
While the fight took place in Wroclaw, Poland, it was virtually a home fight for Usyk as a third of the 40,000 crowd had travelled from Ukraine or were refugees now living in Poland or neighbouring countries because of Russia's invasion of Ukraine.
Dubois' promoter Frank Warren called it a "complete home decision", as Usyk went on to stretch his unbeaten professional record to 21 fights and retain his WBA 'Super', WBO and IBF titles.
Warren plans to lobby the WBA to change the result to a no-contest or order an immediate rematch.
"I definitely want a rematch - get justice, get a rematch, get something to happen," Dubois added.
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